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viability ghost dye  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences viability ghost dye
    Viability Ghost Dye, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 539 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ghost+dye+violet+510/Ghost+Dye+Violet+510/pm41932810-297-4-7
    Average 96 stars, based on 539 article reviews
    viability ghost dye - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Albumin-Bound STING Agonist Reprograms HSPCs to Antitumor Neutrophils Enhancing CD8 + T Cell Immunity.
    Article Snippet: .. Cells were stained with Ghost Dye Violet 510 (30 min, 4◦C), blocked with anti-CD16/32 (10 min, 4◦C), then stained with fluorochrome-conjugated antibodies (Table S1 ) for 20 min at 4◦C, washed, fixed (BioLegend), and analyzed on a Cytek Aurora cytometer; data were processed using FlowJo (Figure S22 ). .. For HSPC analysis, bone marrow single-cell suspensions were enriched for lineage-negative (Lin− ) cells using a biotinconjugated lineage panel (BioLegend) and magnetic separation, then stained with fluorophore-conjugated antibodies (Table S1 ) for flow cytometric analysis of HSPC subsets (Figure S23 ).

    Article Title: Sustained Delivery of a Shingles Subunit Vaccine Overcomes Age-Related Declines in Humoral and Cellular Immunity Relative to Shingrix
    Article Snippet: .. After lymph node dissociation into single cell suspensions, staining for viability was performed using Ghost Dye Violet 510 (Tonbo Biosciences, cat. ..

    Article Title: ImmunoPheno: A Computational Framework for Data-Driven Design and Analysis of Immunophenotyping Experiments
    Article Snippet: Cryopreserved peripheral blood mononuclear cells (PBMCs; STEMCELL Technologies) were thawed in R10 medium (RPMI-1640 (Corning) supplemented with L-glutamine and 10% FBS (HyClone)) on the day prior to sorting and allowed to recover overnight under standard culture conditions (37°C, 5% CO2). .. On the day of sorting, cells were washed twice with PBS (Gibco) and then stained with Ghost Dye Violet 510 (Cytek Biosciences) for 30 min at 4°C in the dark to identify dead cells. .. Cells were then incubated in Human TruStain FcX (BioLegend) for 15 min at 4°C to reduce non-specific antibody binding.

    Article Title: A combinatorial DNA vaccine encoding APC-targeted, bivalent HA and NA antigens results in an improved influenza vaccine.
    Article Snippet: The next day, 5 × 105 preactivated NK cells supplemented with 10 ng/ mL IL-15, 2 μg/mL anti-CD107a (LAMP-1)-PE (1D4B; BioLegend), and GolgiPlug protein transport inhibitor (1:1,000; BD Biosciences) were added. .. After incubation for 5 h at 37 �C and 5% CO2, cells were stained with Ghost Dye Violet 510 (1:400; TONBO) and blocked with 20% rat serum, followed by staining with 5 μg/mL anti-CD3e FITC (145-2C11; TONBO) and 2 μg/mL anti-NKp46 eFluor 450 (29A1.4; Thermo Fisher Scientific). .. Cells were fixed in 3% formaldehyde, permeabilized with Perm/Wash buffer (BD Biosciences), and stained intracellularly with 5 μg/mL antiIFN-γ APC (XMG1.2; TONBO).

    Article Title: Computational discovery of precision therapeutics for hidradenitis suppurativa
    Article Snippet: .. Following ex vivo culture, samples were then stained extracellularly using Ghost Dye Violet 510 (Tonbo Bioscience, 13- 0870-T100), Brilliant Violet 711 anti-human CD45 Antibody (BioLegend, 304050), PE- CF594 Mouse anti-human CD4 (BD Horizon, 562281), CD3 Monoclonal Antibody APC-eFluor 780 (Invitrogen, 47- 0036- 42), Brilliant Violet 650 anti-human CD8a Antibody (BioLegend, 301042), CD127 PE Clone hIL7R-M21 Cat 557938, CD19 Alexa700 CloneHIB19;Cat:302225, ICOS BV785 Clone 313533 Cat: C398.4A and BD APC Mouse Anti-Human CD25 (BD Biosciences, 340939). .. Fixation and permeabilization was performed using the eBioscience Intracellular Fixation & Permeabilization Buffer Set (Invitrogen, 88-8824-00).

    Article Title: Nociceptor neurons control pollution-mediated neutrophilic asthma
    Article Snippet: The cell pellet was resuspended, subjected to RBC lysis (Cytek, # TNB-4300-L100 or Gibco, # A1049201), and stained for surface markers before flow cytometry. .. Single-cell suspensions derived from BALF or lung samples were stained with Ghost Dye Violet 510 (Cytek, # 13-0870-T100) and appropriate antibody cocktails in PBS. .. Cells were incubated at 4°C for 30 min, then fixed with 10% neutral buffered formalin (Sigma-Aldrich, # HT501128) at room temperature for 15 min before data acquisition.

    Article Title: A combinatorial DNA vaccine encoding APC-targeted, bivalent HA and NA antigens results in an improved influenza vaccine.
    Article Snippet: .. Bound vaccine proteins were detected with 2 μg/mL biotinylated anti-HAPR8 (H36-4-52) followed by streptavidin-PerCP (Biolegend), or anti-NA R-PE (3H03, kindly donated by Prof. Rebecca Cox, University of Bergen, Norway).31 Ghost Dye Violet 510 (1:400; TONBO) was used for Live/Dead staining. .. Cells were analyzed using an Attune NxT flow cytometer (Thermo Fisher Scientific) and FlowJo software version 10.8.1 (BD Biosciences).

    Cytometry:

    Article Title: Albumin-Bound STING Agonist Reprograms HSPCs to Antitumor Neutrophils Enhancing CD8 + T Cell Immunity.
    Article Snippet: .. Cells were stained with Ghost Dye Violet 510 (30 min, 4◦C), blocked with anti-CD16/32 (10 min, 4◦C), then stained with fluorochrome-conjugated antibodies (Table S1 ) for 20 min at 4◦C, washed, fixed (BioLegend), and analyzed on a Cytek Aurora cytometer; data were processed using FlowJo (Figure S22 ). .. For HSPC analysis, bone marrow single-cell suspensions were enriched for lineage-negative (Lin− ) cells using a biotinconjugated lineage panel (BioLegend) and magnetic separation, then stained with fluorophore-conjugated antibodies (Table S1 ) for flow cytometric analysis of HSPC subsets (Figure S23 ).

    Single Cell:

    Article Title: Sustained Delivery of a Shingles Subunit Vaccine Overcomes Age-Related Declines in Humoral and Cellular Immunity Relative to Shingrix
    Article Snippet: .. After lymph node dissociation into single cell suspensions, staining for viability was performed using Ghost Dye Violet 510 (Tonbo Biosciences, cat. ..

    Article Title: Nociceptor neurons control pollution-mediated neutrophilic asthma
    Article Snippet: The cell pellet was resuspended, subjected to RBC lysis (Cytek, # TNB-4300-L100 or Gibco, # A1049201), and stained for surface markers before flow cytometry. .. Single-cell suspensions derived from BALF or lung samples were stained with Ghost Dye Violet 510 (Cytek, # 13-0870-T100) and appropriate antibody cocktails in PBS. .. Cells were incubated at 4°C for 30 min, then fixed with 10% neutral buffered formalin (Sigma-Aldrich, # HT501128) at room temperature for 15 min before data acquisition.

    Incubation:

    Article Title: A combinatorial DNA vaccine encoding APC-targeted, bivalent HA and NA antigens results in an improved influenza vaccine.
    Article Snippet: The next day, 5 × 105 preactivated NK cells supplemented with 10 ng/ mL IL-15, 2 μg/mL anti-CD107a (LAMP-1)-PE (1D4B; BioLegend), and GolgiPlug protein transport inhibitor (1:1,000; BD Biosciences) were added. .. After incubation for 5 h at 37 �C and 5% CO2, cells were stained with Ghost Dye Violet 510 (1:400; TONBO) and blocked with 20% rat serum, followed by staining with 5 μg/mL anti-CD3e FITC (145-2C11; TONBO) and 2 μg/mL anti-NKp46 eFluor 450 (29A1.4; Thermo Fisher Scientific). .. Cells were fixed in 3% formaldehyde, permeabilized with Perm/Wash buffer (BD Biosciences), and stained intracellularly with 5 μg/mL antiIFN-γ APC (XMG1.2; TONBO).

    Ex Vivo:

    Article Title: Computational discovery of precision therapeutics for hidradenitis suppurativa
    Article Snippet: .. Following ex vivo culture, samples were then stained extracellularly using Ghost Dye Violet 510 (Tonbo Bioscience, 13- 0870-T100), Brilliant Violet 711 anti-human CD45 Antibody (BioLegend, 304050), PE- CF594 Mouse anti-human CD4 (BD Horizon, 562281), CD3 Monoclonal Antibody APC-eFluor 780 (Invitrogen, 47- 0036- 42), Brilliant Violet 650 anti-human CD8a Antibody (BioLegend, 301042), CD127 PE Clone hIL7R-M21 Cat 557938, CD19 Alexa700 CloneHIB19;Cat:302225, ICOS BV785 Clone 313533 Cat: C398.4A and BD APC Mouse Anti-Human CD25 (BD Biosciences, 340939). .. Fixation and permeabilization was performed using the eBioscience Intracellular Fixation & Permeabilization Buffer Set (Invitrogen, 88-8824-00).

    Derivative Assay:

    Article Title: Nociceptor neurons control pollution-mediated neutrophilic asthma
    Article Snippet: The cell pellet was resuspended, subjected to RBC lysis (Cytek, # TNB-4300-L100 or Gibco, # A1049201), and stained for surface markers before flow cytometry. .. Single-cell suspensions derived from BALF or lung samples were stained with Ghost Dye Violet 510 (Cytek, # 13-0870-T100) and appropriate antibody cocktails in PBS. .. Cells were incubated at 4°C for 30 min, then fixed with 10% neutral buffered formalin (Sigma-Aldrich, # HT501128) at room temperature for 15 min before data acquisition.



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